A total of 53 native Australian species (52x C3, 1x C4) were sampled from 22 plant families and 7 growth forms along a transect in WA spanning 9.56 degrees latitude and 6.85 degrees longitude. Samples were collected using the nationally-accepted AusPlots Rangelands methodology. Samples were stored to preserve isotopic signatures and analysed using standard techniques for mass spectroscopy, including internationally-calibrated standards. Technical replicates of 13% showed very low drift (0.07).

The data set contains information on topsoil chemistry for 20, 10 cm deep soil cores sampled along an elevation gradient (40-1550 m a.s.l.) in Far North Queensland. Information on soil C:N, N:P and C:P ratios and soil pH and organic matter content are provided. Soil elemental composition such as calcium, potassium, phosphorus, sulphur, iron, manganese, boron, aluminum, copper, zinc, lead, chromium, and cadmium are also provided. In addition, the data set contains information on soil δ¹³C and δ¹⁵N isotope concentration.

The record contains information on leaf trait and stable isotope data of <i>Eucalyptus salubris</i> trees in the Credo Flux tower area, from the Great Western Woodlands Site. Data on individual tree height, stem circumference and leaf traits such as leaf thickness, leaf mass, leaf density, specific leaf area, leaf chemical data, including the d¹³C and d¹⁵N content are provided. In addition, data on soil chemical analysis from the site are provided.