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The dataset comprises of a biological and a spatial component. Biological data: This was collated from several sources, collected over the period 2000-2009. Data are lists of presence-absence of 215 native plant species (i.e., exotic species removed) from 76 seasonal wetlands (size range 0.5 - 35 ha) located on the Fleurieu Peninsula, South Australia (centred on latitude 35.5 °S). After data were collated into a single dataset, sampling bias was removed to create a dataset of near-complete census wetlands. Spatial data: For each of the 76 wetlands a small amount of spatial data is also provided, i.e., area, centroids, catchment etc. The dataset could be of interest for any typical community data analysis (e.g. beta diversity, similarity, assembly)- provided only native wetland plant species are of interest. Data presented here were used to model extinction risk, species-area relationships, occupancy distributions and so on.