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The dataset contains information from the first initial trial of the AusPlots Fauna Protocol conducted at Calperum Station, Renmark, South Australia. Selected proposed methodologies and fauna survey techniques were trialled for logistical purposes. After the field trials, the proposed methodologies and techniques were refined. The dataset contains species information on fauna species captures, observations, and specimen collections from the April-May 2015 field trials. The data can be used to review the outcomes of the survey methodologies, presence data of the species recorded, morphological details of the animals recorded, and relate field data to the whole specimen and tissue specimens collected. The Enhancing Long-term Surveillance Monitoring Across Australia Programme will enhance the breadth and depth of Australia's terrestrial ecosystem condition monitoring and reporting at national and regional scales through building on the Terrestrial Ecosystem Research Network (TERN) AusPlots Facility. Specifically, this will be achieved by increasing the range and type of AusPlots field sites and monitoring, and through providing guidelines, protocols manuals or standards that will enhance environmental data quality.