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The project is focused on the topic, 'enhanced heat tolerance of virus-infected aphids lead to niche expansion and reduced interspecific competition. The two aphid species studied are <i>Rhopalosiphum padi</i> and <i>Rhopalosiphum maidis</i>. The project had some of the following objectives: [1] Spatial distribution of two aphid species on the host plants [2] Upper thermal limits of two aphid species. [3] Effects of the viral infection on the host plant thermal profile. [4] Levels of expression of heat shock protein genes of virus-free and viruliferous aphids. [5] Locomotor capacity of aphids, effects of viruses on the locomotor capacity. [6] Effects of viral infection, temperature, and competition on the lifespan and fecundity of <i>R. padi</i> [7] Effects of viral infection, temperature, and competition on the lifespan and fecundity of <i>R. maidis</i> [8] Temperature of acrylic tubes used on aphid experiments. [9] Thermal lethal dose 50 of virus-free and viruliferous aphids [10] Thermal preference of virus-free and viruliferous aphids. This information can be very useful for ecologist working on insect population dynamics as well as physiologist and eco-physiologists doing meta-analyses of expression of heat shock protein genes induced by symbionts.