This dataset provides understorey herbaceous biomass, ground cover and overstorey woody cover response to different fire regimes over a twenty year period at a grassland and open woodland in the tropical savannas of northern Australia. BOTANAL was used to assess understorey herbaceous biomass. Woody canopy cover was derived from digital analysis of oblique aerial imagery taken from a helicopter at the site in 1995 and again in 2013. Woody cover (tree basal area and canopy cover) was also assessed using a bitterlich gauge on BOTANAL ground based transects in 2009. The data could be used to calibrate models of herbaceous growth and woody cover change in response to long term fire. It may be useful for assessing climate change impacts on aboveground carbon sequestration. The fire regimes tested were of varying frequency (every 2, 4 and 6 years) and season (June vs. October) of fire compared to unburnt controls on woody cover and pasture composition. Sites were open to grazing by cattle.

The qualities of these data include: (i) sound experimental design to detect a change between confounding factors, (ii) large sample size, (iii) microchipped animals, (iv) validated heamatological processing on the wild Australian lizard Tiliqua rugosa involving a collaboration between wildlife ecologists and veterinary scientists. Its reuse potential may involve a comparative analysis of body size, haematological parameters with other long-lived, medium-sized lizards, ectoparasite studies (Aponomma hydrosauri, Amblyomma libatum) for different host populations, and background justification for ecotoxicological (pesticide) studies in farmland. Using a using a multivariate, one-way nested Type I PERMANCOVA (analysis of covariance) design, body size, blood samples and ectoparasite presence was collected on a total of 119 animals from two different populations in southern Australia. One population was from an intensively managed cropping environment and one was from an adjacent a less intensively managed grazing environment. This study took place in extensive rangelands and the fragmented landscapes of the South Australian Murray Mallee cereal cropland in southern Australia. Adult and juvenile T. rugosa were captured for sampling at one rangeland (baseline) site and three severely modified (severe) landscape-scaled sites (LS1, LS2, LS3) over a large area (68 km × 84 km or 571,200 ha) across the croplands. Two animal sampling designs were used to collect data on physiological health (Design 1: Baseline vs Severe and Design 2 - Severe only). Data collected: Record No., Animal No., Treatment, Habitat Type, Landscape No., Connectivity Class, Age Class, Linear Body Size Index (LBSI), Heterophil (H) Field of View, Heterophil per microlitre, Total White Blood Cell Count, Absolute Heterophil Count, % Heterophil Count, Absolute Lymphocyte (L) Count, % Lymphocytes, H:L Ratio (Absolute), H:L Ratio (%), Absolute Monocytes, % Monocytes , Absolute Other Granulocytes , % Other Granulocytes, % Polychromasia, Snout-Vent Length (mm), Total No. Ectoparasites per Animal.