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River sites were sampled during the summers of 2008/09 and 2009/10 in a survey designed to identify correlations between commonly used river condition variables and grazing land-use. Potential stream sites in northern Tasmania were screened by catchment size, northing and slope, and according to attributes aimed at minimising confounding variables, maintaining broad consistency in landscape and geomorphological context, and promoting independence among sites. A set of 27 survey sites was selected across a gradient from low to high proportion of land under grazing in their upstream catchments. Catchment sizes varied from 20-120 km2 and proportion grazing from 0-80%. Macroinvertebrates were sampled using Surber sampler. All macroinvertebrates within a 20% sub-sample identified to family and counted, with individuals from the insect orders Ephemeroptera, Plecoptera and Trichoptera identified to genus/species (by Laurie Cook, UTAS). Algal abundance was estimated at each site as the proportion of algal cover and as areal density of benthic chlorophyll a. Physical data variables collected were: water temperature, conductivity, turbidity, pH, total alkalinity, nitrate+nitrate, dissolved reactive phosphorus, total nitrogen, total phosphorus, overhead shading, the proportion of fine sediments within the sampled riffle zone, accumulated abstraction index and accumulated regulation index. For more information see: See Magierowski RH, Read SM, Carter SJB, Warfe DM, Cook LS, Lefroy EC and Davies PE. Inferring landscape-scale land-use impacts on rivers using data from mesocosm experiments and artificial neural networks. PLOS ONE.